Single-cell Proteomics by Mass Cytometry

We are a National facility providing state-of-the art Mass Cytometry (CyTOF™, Fluidigm Inc.) analysis to the entire Swedish research community. The facility consists of two nodes - Stockholm and Linköping. At Stockholm node - we are based at Scilifelab where we provide state-of-the-art Mass cytometry analysis to the entire Swedish and non-Swedish research community. Standardized Mass cytometry experiments on customer provided samples are performed using validated panels of metal-coupled antibodies. We also perform technology development of new experimental assays and reagents as well as novel data analysis tools

Mass Cytometry

Mass Cytometry (CyTOF) is one of the most powerful and advanced single cell technologies that enables high-parameter studies, where comprehensive phenotyping and functional profiling of cells is performed with the ability to measure greater than 40 markers per cell as compared to traditional fluorescence-based cytometry. It helps in systems level understanding of biology in health and disease. Cellular targets are labeled with metal-tagged antibodies that are detected and quantified in individual cells in suspension using CyTOF2™ (Fluidigm) which is based on an Inductively-Coupled Plasma - Mass Spectrometer (ICP-MS) . Samples are acquired at a rate of about 500-1000 cells/sec. The high purity and choice of metal isotopes provide minimal background noise from signal overlap.

Selected References

Bandura, D.R. et al. “Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry.” Anal Chem 81(16) (2009): 6813–6822.

Majonis, D. et al. “Synthesis of a functional metal-chelating polymer and steps toward quantitative mass cytometry bioassays.” Anal Chem (2010).

Ornatsky, O. et al. “Highly multiparametric analysis by mass cytometry.” J Immunol Methods 361(1–2) (2010): 1–20

Bendall, S.C. et al. “Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.” Science 332(6030) (2011): 687–696.

Apply for a Mass Cytometry Service Project

Researchers with an interest in understanding the cellular frequencies and functional states in their own samples of interest, are kindly invited to apply for a Mass cytometry service project using our web application form Application Portal

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Sample Preparation for Mass Cytometry (CyTOF)

Human & Mouse samples

Human Peripheral blood mononuclear cells (PBMCs) or mouse cell suspension should be frozen using a cryoprotective media containing 10% dimethyl sulfoxide (DMSO) and 90% fetal bovine serum (FBS) and stored in -80oC or Liquid Nitrogen. The samples should be transported (locally)/shipped (outside Stockholm) in a box with Dry ice or Liquid Nitrogen containers to our facility.

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Validated Antibody Panels

Mass cytometry provides a powerful way to better understand heterogeneous cell suspensions, like whole-blood or peripheral blood mononuclear cells (PBMC) samples. Both mouse and human cells can be analyzed using one of our validated antibody panels.

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Contact Us for Suggestions

We are continuously working to increase the number of antibody panels validated for service projects and to better cover the interests of researchers. To suggest novel antibody panels and experiments for service project, please use the contact form.